COURSE OBJECTIVES

This advance core course covers the analysis of sequences both in prokaryotes and eukaryotes and also make students able to infer the information from the sequences. Various state of the art techniques for proteome analysis are taught. The course also focuses on application of Bioinformatics for analysis of genome sequences and proteome analysis.

COURSE LEARNING OUTCOMES (CLO)

CLO: 1. Analyze various analytical methods for Genomics
CLO: 2. Comprehend basic techniques used for proteome analysis
CLO: 3. Application of Bioinformatics and molecular Biology techniques used for analysis of genomes and proteomes


COURSE CONTENT

  • Organization and structure of genomes, Artificial chromosomes, Synthetic Biology, Epigenetics
  • Genetic Mapping (RFLP, Microsatellite, SNP, STS, EST), Genetic interaction Mapping (DNA DNA interaction, DNA protein interactions)
  • Genetic Mapping (RFLP, Microsatellite, SNP, STS, EST), Genetic interaction Mapping (DNA DNA interaction, DNA protein interactions)
  • DNA Sequencing Strategies (Manual to NGS) epigenomic sequencing
  • Structural and Functional Annotation
  • Metagenomic analysis, Structural variations in genome and its applications
  • Transcriptomics and Gene expression analysis (Microarray, SAGE) • RNA sequencing (Single Cell Omics)
  • Massively parallel reporter assay (MPRAs)
  • Proteomics, Cellular Communication and Signaling pathways
  • Protein Protein Interaction, Yeast Two Hybrid system
  • Proteomics Analysis (AP-MS, TAP, FRET), Deep mutational Screening
  • Loss of function techniques (Mutagenesis, Somatic Cell and Radiation Hybrids)
  • miRNA and RNA
  • Crisper-Cas
  • Consortium projects on Functional Genomics (ENCODE, GTEx projects)