Biochemistry Lab (BS2221)

The Introductory Biochemistry course covers fundamental biochemical and molecular biological laboratory techniques, supporting concepts, and data analysis. The aims of this course are to provide students with practical knowledge and hands-on experience with some of the most common experimental methods used in biochemical and molecular biological research. Methods include reagent preparation, proper use of instrumentation, biochemical analysis, molecular biology techniques, and utilizing computers to analyze and present data.

CLO: 1. Understand safety measures, concepts and the investigative procedures of Biochemistry Lab.
CLO: 2. Differentiate between various biomolecules using biochemical analysis
CLO: 3. Be able to display good interpersonal communication and act as an effective team member by completing assigned Lab tasks

1 Introduction to the Laboratory and safety Protocols
2 Use of Pipetman
3 Introduction Lab Equipment
4 Solution Preparation and Dilutions
5 Test for Carbohydrates
6 Protein Biochemistry
7 Determination of the isoelectric point
8 SDS PAGE-1
9 Mid Exam
10 Lipids profile-1
11 Lipids profile-2
12 Nucleic acids-1
13 Spectrophotometry
14 PCR
15 Agarose Gel electrophoresis