Molecular Genetics

Cell Biology (BI-1113)

Biology, 2nd Edition By Brooker/McGraw-Hill, ISBN 978-0077349967

Molecular Biology, Howell, Stephen H (Ed.),  2014 Springer-Verlag New York

Molecular genetics addresses the structure, function, and regulation of the molecules involved in heredity – DNA, RNA, and proteins. We will discuss many genetic applications and issues including genetic engineering, gene therapy, molecular medicines, eugenics, and biotechnology. In the laboratory, we will learn numerous molecular techniques including DNA purification, PCR, gene cloning, and recombinant DNA expression using a viruses.

CLO: 1. Understand Basic of central dogma of molecular Biology

CLO: 2. Understand basic structure and function of DNA and RNA

CLO: 3. Understand the process of replication in prokaryotes and eukaryotes

CLO: 4. Understand basic of gene expression and regulation

CLO: 5. Introduction to the recent applications of Molecular Biology concepts

• Nucleic acids as genetic material,
• structure of A-, B- and Z-DNA,
• palindromic sequences,
• structure of RNA (t-RNA, m-RNA and r-RNA),
• DNA denaturation and renaturation.
•  DNA replication in prokaryotes,
•  DNA polymerase I, II and III,
• modes and mechanism of DNA replication,
• DNA repair mechanisms.
• Transcription in prokaryotes,
• RNA polymerase,
• Types and functions of RNA polymerases in eukaryotes.
• Genetic code,
• translation in prokaryotes,
• Post-translational modifications.
•  Gene organization,
• Operon concept and introduction to gene regulation mechanisms.
• Transposons:
•  An elementary idea Basic concept of recombinant DNA technology,
• principles of gene cloning.
• Restriction modification systems, use of restriction enzymes in biotechnology, cloning vectors.
  • Methods of gene transfer, DNA libraries. Introduction to PCR, RFLP, DNA sequencing, blotting techniques.