1.To understand different sterilization techniques and bacterial growth curve
2.To demonstratee different antibiotics susceptibility testing approaches to understand the resistance and sensitivity pattern of different microorganisms.
3.To acquire knowledge about some staining techniques and sterility testing.


CLO: 1. Describe the basic microbiological laboratory approaches used for sterilization and staining techniques.
CLO: 2. Illustrate the study of microbiology of various bacteria from different sources, its detection and identification.
CLO: 3. Apply the different approaches of antibiotic susceptibility testing and antibiotics isolation from different sources.


1. Study of physical methods of sterilization.
2. Study of chemical methods of sterilization.
3. Preparation of bacterial spores’ film and spores staining.
4. Preparation of McFarland turbidity standards.
5. Study of bacterial growth curve by plotting absorbance vs. time graph.
6. Study of microbiology of soil.
7. Isolation of antibiotic producing organisms from soil.
8. Antibiotic susceptibility testisng by agar well diffusion method.
9. Antibiotic susceptibility testing by disc diffusion method.
10. Determination of minimum inhibitory concentration (MIC) of an antibiotic by tube dilution method.
11. Differentiate bactericidal vs. bacteriostatic activity of antibiotics.
12. Enumeration of microorganisms by viable count method.
13. Sterility testing for parenteral dosage forms
14. Detection of microorganisms in the given sample by Giemsa staining.
15. Exploration and identification of different antibiotics producing strains in soil, air and water.
16. Determination of antibacterial activity of different herbal extracts.