COURSE OBJECTIVES

1.To understand the principles of animal handing and drug administration.
2.To demonstratee pharmacological action of a drug in a laboratory setting to develop practical understanding of the drug action on laboratory animals.
3.To understand possible underlying mechanism/receptor involvement and illustration.

COURSE LEARNING OUTCOMES (CLO)

CLO: 1. Describe action of a given drug on laboratory animals
CLO: 2. Demonstrate mechanism of action of drugs using agonist/antagonist
CLO: 3. Analyze the given data and interpret the results

COURSE CONTENTS


1. Laboratory protocols
2. Handling of laboratory animals
3. Introduction to central nervous system and neurotransmitters.
4. Determination of convulsant effect of drugs in frog and its site of action
5. Determination of convulsant effect of nikethamide in frog and determine its site of action
6. Determination of convulsant effect of caffeine in frog and determine its site of action
7. Determination of convulsant effect of strychnine in frog and determine its site of action
8. Determination of effect of CNS depressant (Diazepam) in frog
9. Determination of effect of CNS depressant (MgSO4) in frog
10. Determination of analgesic activity of tramadol in albino mice
11. Determination of analgesic activity of diclofenac sodium in albino mice
12. Elucidation of mechanism of action of tramadol morphine by acetic acid induced writhing method
13. Determination of effect of local aesthetic on rabbit eye