Introduction to Immunology Lab (BS1151)

The overall objective of this course is to give the advanced biology students experience with addressing immunological questions through experimentation. A representative experiment will be the analysis of different lymphocyte subsets in distinct lymphoid tissues of the mouse. Specific techniques will analyze RNA and protein expression in different immune cell populations and explore the functions of these unique cells

CLO: 1. Understand safety measures, concepts and the investigative procedures of Immunology Lab.
CLO: 2. Comprehend the basic of Immunological technique.
CLO: 3. Be able to display good interpersonal communication and act as an effective team member by completing assigned Lab tasks

1 Laboratory Safety Guidelines
2 Blood Grouping
3 Blood Smear Preparation
4 Blood Collection
5 Course Profile for immunology Lab
6 ELISA
7 Instruction for solution preparation lab
8 Isolation of lymphocytes
9 Mid Exam
10 RBC Count and WBC count
11 SDS PAGE
12 Double diffusion assay
13 PCR
14 Biosafety levels
15 Biosafety Cabinets