COURSE OBJECTIVES

The course covers the chemistry of proteins and their modifications that promote regulation of cellular and molecular transformations in biological systems.

COURSE LEARNING OUTCOMES (CLO)

CLO: 1. Understand the different classifications of protein structure, and how these structural elements affect the overall structure [C1-Comprehension]
CLO: 2. Design experimental approaches to protein engineering and expression [C2-Analysis].
CLO: 3. Suggest strategies for analyzing a mixture of proteins using modern protein analytical
Instruments [C4-Current tool usage

COURSE CONTENTS

1 Introduction to Protein chemistry
2 Amino acids Classification
3 Structure of Protein
4 Structure of Protein
5 Classification of Protein
6 Protein folding
7 Protein folding
8 Protein Modification & Cleavage
9 Protein Separation & Purification techniques Introduction
10 Dialysis, Gel-Filtration Chromatography
11 Ion-Exchange Chromatography
12 Affinity Chromatography
13 High Performance liquid chromatography
14 Gel Electrophoresis
15 SDS PAGE, western blotting
16 Mass Spectrometry