dr.shaukat

Dr. Shaukat Iqbal (PhD NKUA, Athens)

PROFESSOR
PROFILE SUMMARY

My research interests are G-2 Assays, (PCC) SCEs and Cytogenetic Techniques to determine Carcinogenicity & Genotoxicity from Chemical used in Agriculture, Medical Sciences and Environment, (e.g. Mutagens). Cytogenetic is also used for the detection for Leukemia, Genetic Disorders and Prenatal Diagnosis.

I loved to study, discuss and conduct research in biological sciences and to evaluate the root cause of genetic abnormalities. I have engaged the last 20 years of my life to get advance knowledge, to teach and to conduct research in the domain of genetics and Bio-chem y. I have published several papers in the different area of genetic anomalies including leukemia, radio-sensitivity, chromosomal aberrations, and several other genetics problems lead to severe human diseases.

As a professional, for the successful delivery of knowledge I, strongly believe on creative teaching, Innovative approaches and being interactive all the time during the lecture. In addition to that I have presented scientific base talk in several national and international conferences and symposia. I have introduced G2 Phase cytogenetic technique that was published in Oxford University in its Journal of Mutation.

Currently, we investigate Falcarinol Type polyacetylenes of Carrot inhibit proliferation and induce apoptosis in hepatocarinoma cell line, Hormonal Fluctuation of Postmenopausal Risk Leading to Breast Cancer in Pakistani population. Calcium dose involve in disrupting of cell cycle leading cancer in breast cells, Chromosomal Linkage with HIV patients in addition to that we are working on looking different aspect of Breast Cancer in Pakistani population.

QUALIFICATION
2nd Postdoctoral Cancer Cytogenetics Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, (LBCC, UNC-CH) NC.USA 2009
1st Postdoctoral Cancer Biology US Environmental Protection Agency , NHEERL, Cancer Biology Branch (Chemical Cytogenetics Section) RTP, NC, USA 2008
PhD Biochemistry and Molecular Biology National & Kapodestrain University of Athens & Cancer Cytogenetics & Environmental Hygiene Laboratory, NCSR “Demokritos, Athens 2004
M.Sc (Hon’s) Cytogenetics Sindh University, Pakistan 1998
B.Sc (Hon’s) Genetics Agricultural University Faisalabad, Pakistan 1993
TEACHING EXPERIENCE
Professor Capital University of Science and Technology (CUST), Islamabad Since – 2018
Associate Professor Capital University of Science and Technology (CUST), Islamabad 2015 – 2018
Associate Professor Qatar University 2014
Associate Professor National University of Science & Technology, Islamabad 2012 – 2013
Associate Professor Mohammad Ali Jinnah University, Islamabad 2007 – 2011
MANAGEMENT EXPERIENCE
Director VIS Directorate, Capital University of Science & Technology, Islamabad 2018 – 2021
Convener CUST university Discipline Committee 2017
Head Research of PG & UG Lab in CUST Since – 2016
Acting Chairman BUTUMS 2004 – 2005
Manager Animal Lab. House in NUST 2012 – 2013
Member NTS subject committee 2005 – 2007
Member Admission Subject Committee, NUST 2012 – 2013
Member Selection Board at NUST 2012 – 2013
HONORS & AWARDS
1. Selected for interview Fulbright Postdoc scholarship in 2014 but not availed due to Qatar University, Assignment
2. Students Feed Back: Gained in Qatar University 92. % PG & 78.6 % student’s feedback 2014 in advance bioinformatics Course.
3. Students Feed Back : in NUST 90.8% PG & 79.2 % students feedback
4. Best Faculty Performance Award Received Rs.30, 000 Cash from BUIETMS, ( 2005)
5. In 2007 HEC has awarded a Research Project under NRPU entitled “Genotoxicity testing of pesticides, plant protection products by means of pre mature chromosome condensation(PCC) in peripheral blood lymphocytes” Not Released yet, amount: PkR.3,703,920/-
6. Earned Excellent Professor award year09-10&10-11 in M.A. Jinnah University, Isb
7. Earned Scholarship from The Jackson Laboratory Medical
8. Scholarship for Experimental Models of Human Cancer
9. Earned Postdoc fellowship from HEC, Pakistan in 2008, for USA
10. Ten Times HEC Travel grants Received for paper presenting in International Conferences.
11. Several Lecture deliver as invited speaker in different DAI in Pakistan and abroad
12. Earned Scholarship for PhD from European Union DAC program via Greek Ministry of Education and Religious Affairs, Athens
13. Earned Scholarships for M. Sc (Hon’s)
14. Earned Scholarship for B. Sc (Hon’s) from Government of Baluchistan
RESEARCH AREAS / INTERESTS

1. Molecular Biology & Human Genetics;
2. Cancer Cytogenetics;
3. Comet Assay;
4. Micro-arrays Gene Expression & Data Analysis

RESEARCH SUPERVISION
1. Ph.D. Cytogenetic approach for the evaluation of mutagenic potential causes cancer
2. Ph.D. Molecular Characterization and Computational Analysis of Drug-Resistant Strains of Mycobacterium Tuberculosis
3. Ph.D. Prevalence of Different Cancer in Pakistani Population and their Causes
4. Ph.D. Molecular Characterization and Computational Analysis of Drug-Resistant Strains in Cancer
5. Ph.D. Causes of early development of Bladder Cancer in Pakistani Population
6. M.S. Prevalence of Brest Cancer in local population
7. M.S. Computational Analysis on the Role of GPM6A in Human Thyroid Cancer
8. M.S. In Silico Mining of RAS Gene Family Expression Data in Human Thyroid Cancer
9. M.S. Estrogen Fluctuation of Postmenopausal Risk to Breast Cancer in Pakistani Population
10. M.S. Breast Cancer Prevalence in twin cities of Pakistan
11. M.S. Genetic Analysis of Hereditary Polydactyly in Pakistani Families
12. M.S. Antimicrobial Susceptibility Pattern and Multidrug Resistance Status of Salmonella typhii in population of Rawalpindi
13. M.S. Clinical and Demographics Variables of Beta Thalassemia patients from Islamabad
14. M.S. Assessment of awareness concerning prophylaxis against COVID-19 in Pakistani population
15. M.S. Comparison of Spread of Covid-19 in Various Countries
16. M.S. Isolation and Antibacterial Activity of Bacteriocin Produced by Bacteria Isolated from Yogurt
17. M.S. Association of Interleukin (IL) Il-17A and IL-6 Gene Polymorphisms with Susceptibility to Tuberculosis
18. M.S. Dairy Probiotics: Analysis of their Microbiological and Functional Aspects.
19. M.S. Association of FTO gene polymorphisms with risk of type 2 diabetes: an updated meta-analysis
20. M.S. Interleukin 17a, 17F and 18 SNPs assosiation with asthma: a updated meta-analysis
21. M.S. Association of cytokine gene polymorphisms with the risk of cystic fibrosis: meta-analysis
22. M.S. Relationship among single nucleotide polymorphism of interleukin-4 and interleukin-18 and susceptibility to pulmonary tuberculosis in Pakistani population
23. M.S. Comparison of Biological evaluation of two different varieties of Cumin Seed Extracts
24. M.S. Correlation Between Social Stress Causing Factors (SSCFs) and Appetite Among the Students of Rawalpindi and Islamabad.
25. M.S. Association of TNF-α -308G/A gene polymorphism with the risk of Autoimmune diseases
26. M.S. Identify genetic loci influencing the recovery process using accurate phenotyping to produce the largest genome-wide association study in Atherosclerosis stroke recovery.
27. M.S. Biological Evaluation and Comparison Of Nigella sativa and Trachyspermum ammi.
28. M.S. Association of IL-1β and IL-17F gene polymorphism with susceptibility to TB patients in Pakistan
29. M.S. Causes and investigation of bladder cancer development in preliminary ages of Pakistan and their comparison with European countries
30. M.S. Use of low laser therapy to treat pain in various body parts focused on knee problem
JOURNAL PUBLICATIONS
1. Proliferative effects of lung cancer cells derived exosomes on T cells. Brazilian Archives of Biology and Technology. S Hussain Shaukat I Malik
2. Effects of tumor derived exosomes on T cells markers expression Brazi J Biol. doi: / 10.15901519-6984.250556. S Hussain Shaukat I Malik
3. Prediction of Secondary and Tertiary Structure and Docking of Rb1WT and R b1R661W Protein, Aimen Sajid, M.Shaoor Saeed, Rabbiah M.Malik,Sahar Fazal Shaukat Malik,Kamal, Current Biotechnology.
4. Comparison of Nigella sativa and Trachyspermum ammi via experimental investigation and biotechnological potential. Sadia Arif , Hira Saqib Muhammad Mubashir Shaukat Iqbal Malik, Ahmad Mukhtar Sidra Saqib , Sami Ullah Pau Loke Show , chemical Engineering and Processing – Process Intensification 161 10831
5. Identification of differentially expressed genes and pathways crosstalk analysis in Rheumatoid and Osteoarthritis using next-generation sequencing and protein-protein networks. Qiu S, Munir A, Malik SI, Khan S, Hassan A. Saudi J Biol Sci.28(8):4656-4663. doi: 10.1016/j
6. Isolation and Characterization of Leishmanial AdenineAminohydrolase as a Drug Target.Butt F, Yasinzai M, Malik SI, Munir A. Curr Comput
Aided Drug ;17(7):905-15. doi:10.2174/1573409916666201207194815
7. The Biological importance of cells secreted Exosomes. Hussain S, Fatima A, Fan Malik Shaukat I. Pak J Pharm Sci.; 34(6):2273-2279.3
8. The role of exosomes derived miRNAs in cancer. Hussain S, Zahra Bokhari SE, Fan XX, Malik SI. J Pak Med Assoc. 71(7):1856-1861. doi: 10.47391/JPMA.398.
9. De-Novo Ligand Design against Mutated Huntington Gene by Ligandbased Pharmacophore Modeling Approach. Munir A, Malik SI, Malik
KA. Curr Comput Aided Drug D16(2):134-44.doi10.2174/157340991
10. Current strategies against COVID-19 Shahid Hussain, Ya-Jia Xie Dan Li, Shaukat Iqbal Malik Jin-cai Hou Elaine Lai-Han Leung ,Xing-Xing Fa ,Chinese Medicine
11. Characterization and synthetic biology of lipase from Bacillus amyloliquefaciens strain. Khan MT, Kaushik AC, Rana QUA, Malik SI, Khan AS, Wei DQ, Sajjad W, Ahmad S, Ali S, Ameenullah, Irfan M. Arch Microbiol. (6):1497-1506. doi: 10.1007/s00203-020-01869-0.
12. Artificial Neural Networks for Prediction of Tuberculosis DiseaseKhan MT, Kaushik AC, Ji L, Malik SI, Ali S, Wei DQ. Front Microbiology
13. Pyrazinamide resistance and mutations in pncA among isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan. Khan MT, Malik SI, Ali S, Masood N, Nadeem T, Khan AS, Afzal MT. BMC Infect Dis. 6;19(1):116. doi: 10.1186/s12879-019-3764-2
14. Structural dynamics behind variants in pyrazinamidase & pyrazinamide resistance. Khan MT, Malik SI. J Biomol Struct Dyn. ;38(10):3003- 3017. doi: 10.1080/07391102
15. Insight into novel clinical mutants of RpsA-S324F, E325K, and G341R of Mycobacterium tuberculosis associated with pyrazinamide resistance. Khan MT, Rehaman AU, Junaid M, Malik SI, Wei DQ. Comput Struct Biotechnol J. 4;16:379-387. doi: 10.1016/
16. Pyrazinamide resistance and mutations L19R, R140H, and E144K in Pyrazinamidase of Mycobacterium tuberculosis. Khan MT, Junaid M, Mao X, Wang Y, Hussain A, Malik SI, Wei DQ. J Cell Biochem.doi: 10.1002/jcb.27989.
17. Pyrazinamide-resistant mycobacterium tuberculosis isolates from Khyber Pakhtunkhwa and rpsA mutations. Khan MT, Malik SI, Bhatti AI, Ali S, Khan AS, Zeb MT, Nadeem T, Fazal S. J Biol Regul Homeost Agents. 32(3):705-709.
18. Proteome Mining for the Identification of Putative Drug Targets for Human Pathogen Clostridium TetanI ” Current Bioinformatics,, 13, 1-8 A.Munir, Malik I.S, K Akhtar
19. SARS-CoV-2 nucleocapsid and Nsp3 binding: an in silico study. Khan MT, Zeb MT, Ahsan H, Ahmed A, Ali A, Akhtar K, Malik SI, Cui Z, Ali S, Khan AS, Ahmad M, Wei DQ, Irfan M. Arch Microbiol. 203 (1):59-66. doi: 10.1007/s00203-020-01998-6.
20. Medicinal Plants are Effective Inhibitors of Type I and II Diabetes Anum Munir , Shaukat Iqbal Malik1* , Sehar Aslam , Azhar Mehmood, Saeed Amjad, K. A. Malik, Muhammad Younis, Abbas Husain Shah, G. Mujtaba Shah Pharmacophore, 9(5), P: 1-7 Munir, Malik I.S, K Akhtar
21. Exploring the Pyrazinamide Drug Resistance Mechanism of Clinical Mutants T370P and W403G in Ribosomal Protein S1 of Mycobacterium tuberculosis. Rehman AU, Khan MT, Liu H, Wadood A, Malik Shaukat I, Chen HF. J Chem Inf Model. 22;59(4):1584-97.doi: 10.1021
22. Current strategies against COVID-19. Hussain S, Xie YJ, Li D, Malik SI, Hou JC, Leung EL, Fan X. Chinise Medicine ;15:70. doi: 10.1186/
23. Structural dynamics behind variants in pyrazinamidase & pyrazinamide resistance. Khan MT, Malik SI. J Biomol Struct Dyn. ;38(10):3003-17. doi: 10.1080/0739
24. Gibbs Free Energy Calculation of Mutation in PncA and RpsA Associated With Pyrazinamide Resistance. Khan MT, Ali S, Zeb MT, Kaushik AC, Malik SI, Wei DQ. Front Mol Biosci.9;7:52.doi: 0.3389
25. Artificial Neural Networks for Prediction of Tuberculosis Disease .M.Tahir Khan ,Aman C. Kaushik , Linxiang Ji , Shaukat Iqbal Malik , S. Ali , Dong-Qing Wei Front Microbiol 4;10:395.
26. Structural and free energy landscape of novel mutations in ribosomal protein S1 (rpsA) associated with pyrazinamide resistance. Khan MT, Khan A, Rehman AU, Wang Y, Akhtar K, Malik SI, Wei DQ. Sci Rep. 16;9(1):7482. doi: 10.1038/s41598-019-44013-9.
27. Prevalence of Pyrazinamide Resistance in Khyber Pakhtunkhwa, Pakistan. Khan MT, Malik SI, Ali S, S.Khan A, Nadeem T, Zeb MT, Masood N, Afzal MT. Microb Drug Resist.(9):1417-1421. doi: 10.1089
28. Marine Natural Products and Drug Resistance in Latent Tuberculosis. Khan MT, Kaushik AC, Bhatti AI, Zhang YJ, Zhang S, Wei AJ, Malik SI, Wei DQ. Mar Drugs. 26;17(10):549. doi: 10.3390/md17100549.
29. Pyrazinamide resistance and mutations in pncA among isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan. Khan MT, Malik SI, Ali S, Masood N, Nadeem T, Khan AS, Afzal MT. BMC Infect Dis. 6;19(1):116. doi: 10.1186/s12879-019-3764-2.
30. Structural and free energy landscape of novel mutations in ribosomal protein S1 (rpsA) associated with pyrazinamide resistance. Khan MT, Khan A, Rehman AU, Wang Y, Akhtar K, Malik SI, Wei DQ. Sci Rep. 16;9(1):7482. doi: 10.1038/s41598-019-44013-9.
31. Marine Natural Products and Drug Resistance in Latent Tuberculosis. Khan MT, Kaushik AC, Bhatti AI, Zhang YJ, Zhang S, Wei AJ, Malik
SI, Wei DQ. Mar Drugs.26;17(10):549. doi: 10.3390/md17100549.
32. Pyrazinamide resistance and mutations L19R, R140H, and E144K in Pyrazinamidase of Mycobacterium tuberculosis. Khan MT, Junaid M, Mao X, Wang Y, Hussain A, Malik SI, Wei DQ. J Cell Biochem.
33. Insights intothe mechanisms of pyrazinamide resistance of three pyrazin amidase mutantsN11K, P69T and D126N. Junaid M, Khan MT, Malik
SI, Wei DQ. J Chem Inf Model. 27. doi: 10.1021/acs.jcim.8b00525
34. Investigation of Mycobacterium tuberculosis oxidative stress pathway” TKhan, M Malik, Shaukat; Fazal, Sahar; Kaushik, Aman; Sharif, Muhammad; Irfan, M.; junaid, M.; Wei, DongQing; Bhatti, Aamer Journal:ACS Combinatorial Science
35. Amplification of Mitochondrial DNA for detection ofPlasmodiumvivax in Balochistan. Shahwani MN, Nisar S, Aleem A, Panezai M, Afridi S,
Malik SI. J Pak Med Assoc. 2017 May;67(5):677-681.
36. Prevention through Employ of Appropriate Diet in Daily Schedule, J Cancer Sci Ther Special Issue, S I. Malik, Sajid Khan
37. Candidate Genes Prediction in Pakistani Families with Hearing Impairment by Using Bioinformatics Approach”. Biosci. J. vol. 28, n. 6, p. 1024-1033. T. Noor1; S. Zubai; A. Gul; S.I Malik
38. Computational Analysis on the Role of GPM6A in Human Thyroid Cancer. Journal of Data Mining in Genome Proteomics, Index CopernicusVal:4.55. Z. Khalid1, S. Sameen1, S. I Malik
39. In Silico Mining of RAS Gene Family Expression Data in Human Thyroid Cancer, Published in IEEE Explore. Sameen, S.; Khalid, Z.; Malik, S.I.
40. Lack of association between GSTT1 polymorphism and endogenous or benzo[a]pyrene-induced sister chromatid exchanges as analyzed in metaphase or G2-phase lymphocytes. Hatzi VI, Terzoudi GI, Stavropoulou C, Malik SI, Makropoulos V, Pantelias GE. Mol Biol Rep 38(6):3959-66. doi: 10.1007/s11033-010-0513-4.
41. Role of pyruvate dehydrogenase kinases (PDK’s) and their respective micro RNA’s in human ovarian cancer Journal of Medical Genetics and Genomics Vol. 3(7)115,121. S. Sameen, Z. Khalid1, S. I. Malik
42. In Silico Gene Expression Based Analysis on Claudine Family Members Association with Human Thyroid Cancer. Journal of Proteomics and Bioinformatics, 4:12. Malik S.I, Zoya K, S. Sheema
43. Comparative Homology Modeling of Human Rhodopsin with several templates of Bovine Rhodopsin. African J of Biotechnology Vol. 11(2), pp. 466-473. S. Kanwal, M.Irfan1, Malik S.I, K. Akhtar.
44. Cytogenetic insights into DNA damage and repair of lesions induced by a monomethylated trivalent arsenical. Kligerman AD, Malik SI, Campbell JA. Mutat Res. 2;695(1-2):2-8. doi: 10.1016/
45. SCE analysis in G2 lymphocyte prematurely condensed chromosomes after exposure to atrazine: the non-dose-dependent increase in homologous recombinational events does not support its genotoxic mode of action. Malik SI, Terzoudi GI, Pantelias GE. Cytogenet Genome Res. ;104(1-4):315-9. doi: 10.1159/000077509.
46. A new cytogenetic approach for the evaluation of mutagenic potential of chemicals that induce cell cycle arrest in the G2 phase. Terzoudi GI, Malik SI, Pantelias GE, Margaritis K, Manola K, Makropoulos W. Mutagenesis. ;18(6):539-43. doi: 10.1093
47. Radioprotective effect of amifostine on cells from cancer prone patients and healthy individuals studied by the G2 and PCC assays. Manola KN, Terzoudi GI, Dardoufas CE, Malik SI, Pantelias GE. Int J Radiat Biol.79(10):831-8. doi: 10.1080/09553000310001610222.
48. Lipases of Bacillus amyloliquefaciens strains from Pakistan. M.Tahir Khan#1,2, Shaukat Iqbal Malik, Aman Chandra Kaushik#2, Dong-Qing Wei*2, Muhammad Irfan*4 Khalid Akhtar
CONFERENCE PUBLICATIONS
1. BMS-CCADD-2018-56 ACCEPTED :Title: De-Novo Ligand Design Against Mutated Huntington Gene based on Ligand Based Pharmacophore Modeling Approach , A. Munir, Shaukat I. Malik , K Akhtar
2. BMS-CBIO, 2018-127 accepted “Proteome mining for the identification of putative drug targets for human pathogen clostridium titanium. A. Munir, Shaukat I. Malik ,K Akhtar
3. Pyrazinamide resistance and mutations in pncA among isolates of Mycobacterium tuberculosis from Khyber Pakhtunkhwa, Pakistan. M. Tahir Khan, ; Shaukat I. Malik,; Sajid Ali, ; N Masood, T. Nadeem, Anwar S Khan, ; M. Tanvir Afzal, ACCEPTED in BMC Infectious Diseases
4. Prevalence of Pyrazinamide Resistance in Khyber Pakhtunkhwa, Pakistan. M.Tahir Khan, Shaukat I. Malik, Sajid Ali, Anwar S. Khan, T Nadeem, M. Tariq Zeb, N. Masood, M.T. Afzal. Microbial Drug Resist. 201827. doi:10.1089.2017.0234
5. Manuscript entitled: Elastic network modelling of E7 protein of Human Papilloma Virus and prediction of host proteins affected by E7. VIRUS_2018_151: Corresponding author: Mrs Rabbiah Malik, : Mrs Fakhra Nazir, Shaukat Malik, DSahar Fazal, Mukhtar Ullah, ,Aamer Bhatti
6. “Pyrazinamide resistance and Mutations L19R, R140H and E144K in pncA of Mycobacterium tuberculosis” by Khan, Muhammad; Junaid, muhammad; Malik, Shaukat; Wei, Dong. Submitted manuscript ID number JCB-18-1370.
7. Sameen, S.; Khalid, Z.; Malik, S.I. In Silico Mining of RAS Gene Family Expression Data in Human Thyroid Cancer, Published in IEEE Explore 2011.

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